Journal: Science progress

Article Title: Roles of the CDCA gene family in breast carcinoma.

doi: 10.1177/00368504241312305

Figure Lengend Snippet: Figure 9. Prediction of TFs of CDCAs promoters. (a) Results of EPD and PROMO databases; (b) GEPIA showing up-regulation of FOXP3 and YY1 in BRCA in comparison of normal tissues; (c) correlation between CDCAs and FOXP3 expression in GEPIA; (d) correlation between CDCAs and YY1 expression in GEPIA. *P < 0.05.

Article Snippet: The human BRCA line MDA-MB-231 (FuHeng Biology, China) was cultured at 37 °C with 5% CO2 in a complete DMEM medium (Gibco, USA) containing 10% fetal bovine serum (Gibco, USA). siRNA transfection FOXP3 siRNAs (sc-43569), YY1 siRNAs (sc-36863), and control siRNA (sc-37007) were purchased from Santa Cruz Biotechnology (Santa Cruz, USA).

Techniques: Comparison, Expressing

Journal: Science progress

Article Title: Roles of the CDCA gene family in breast carcinoma.

doi: 10.1177/00368504241312305

Figure Lengend Snippet: Figure 10. Expressions of FOXP3 and YY1 in BRCA samples through IHC: (a) microscopic observation (100×); (b) comparison of FOXP3 and YY1 expression scores between breast tumors and normal tissues (n = 70); (c) correlation between CDCAs and FOXP3 expression in BRCA samples; (d) correlation between CDCAs and YY1 expression in BRCA samples. ***P < 0.001.

Article Snippet: The human BRCA line MDA-MB-231 (FuHeng Biology, China) was cultured at 37 °C with 5% CO2 in a complete DMEM medium (Gibco, USA) containing 10% fetal bovine serum (Gibco, USA). siRNA transfection FOXP3 siRNAs (sc-43569), YY1 siRNAs (sc-36863), and control siRNA (sc-37007) were purchased from Santa Cruz Biotechnology (Santa Cruz, USA).

Techniques: Comparison, Expressing

Journal: Science progress

Article Title: Roles of the CDCA gene family in breast carcinoma.

doi: 10.1177/00368504241312305

Figure Lengend Snippet: Figure 11. Regulation of CDCAs by silencing FOXP3 and YY1:. (a) qRT-PCR investigating the decrease of FOXP3 and YY1 mRNA via siRNAs; (b) Western blotting analysis detecting expressions of FOXP3, YY1 and CDCAs proteins. ***P < 0.001.

Article Snippet: The human BRCA line MDA-MB-231 (FuHeng Biology, China) was cultured at 37 °C with 5% CO2 in a complete DMEM medium (Gibco, USA) containing 10% fetal bovine serum (Gibco, USA). siRNA transfection FOXP3 siRNAs (sc-43569), YY1 siRNAs (sc-36863), and control siRNA (sc-37007) were purchased from Santa Cruz Biotechnology (Santa Cruz, USA).

Techniques: Quantitative RT-PCR, Western Blot

Journal: Science Progress

Article Title: Roles of the CDCA gene family in breast carcinoma

doi: 10.1177/00368504241312305

Figure Lengend Snippet: Prediction of TFs of CDCAs promoters. (a) Results of EPD and PROMO databases; (b) GEPIA showing up-regulation of FOXP3 and YY1 in BRCA in comparison of normal tissues; (c) correlation between CDCAs and FOXP3 expression in GEPIA; (d) correlation between CDCAs and YY1 expression in GEPIA. * P < 0.05.

Article Snippet: FOXP3 siRNAs (sc-43569), YY1 siRNAs (sc-36863), and control siRNA (sc-37007) were purchased from Santa Cruz Biotechnology (Santa Cruz, USA).

Techniques: Comparison, Expressing

Journal: Science Progress

Article Title: Roles of the CDCA gene family in breast carcinoma

doi: 10.1177/00368504241312305

Figure Lengend Snippet: Expressions of FOXP3 and YY1 in BRCA samples through IHC: (a) microscopic observation (100×); (b) comparison of FOXP3 and YY1 expression scores between breast tumors and normal tissues ( n = 70); (c) correlation between CDCAs and FOXP3 expression in BRCA samples; (d) correlation between CDCAs and YY1 expression in BRCA samples. *** P < 0.001.

Article Snippet: FOXP3 siRNAs (sc-43569), YY1 siRNAs (sc-36863), and control siRNA (sc-37007) were purchased from Santa Cruz Biotechnology (Santa Cruz, USA).

Techniques: Comparison, Expressing

Journal: Science Progress

Article Title: Roles of the CDCA gene family in breast carcinoma

doi: 10.1177/00368504241312305

Figure Lengend Snippet: Regulation of CDCAs by silencing FOXP3 and YY1:. (a) qRT-PCR investigating the decrease of FOXP3 and YY1 mRNA via siRNAs; (b) Western blotting analysis detecting expressions of FOXP3, YY1 and CDCAs proteins. *** P < 0.001.

Article Snippet: FOXP3 siRNAs (sc-43569), YY1 siRNAs (sc-36863), and control siRNA (sc-37007) were purchased from Santa Cruz Biotechnology (Santa Cruz, USA).

Techniques: Quantitative RT-PCR, Western Blot

Journal: Frontiers in Immunology

Article Title: Development and validation of immunogenic cell death-related signature for predicting the prognosis and immune landscape of uveal melanoma

doi: 10.3389/fimmu.2022.1037128

Figure Lengend Snippet: Experimental validation of the effect of FOXP3 on uveal melanoma growth. (A) The mRNA levels of Foxp3 in ARPE-19, MUM2B, and OCM-1A cells. (B) The protein levels of FOXP3 in ARPE-19, MUM2B, and OCM-1A cells. (C) Immunoblotting shows the knockdown efficiency of siRNA in MUM2B and OCM-1A cells. (D, E) CCK-8 assays were used to measure the viability of MUM2B (D) and OCM-1A (E) cells after the knockdown of FOXP3. (F, G) The assessment of cell proliferation by using the EdU-DNA synthesis assays following the knockdown of FOXP3. (H) The percentage of Edu-positive cells in (F) was displayed. (I) The percentage of Edu positive cells in (G) was displayed. **P< 0.01, ***P < 0.001, ****P < 0.0001.

Article Snippet: Negative control siRNA (sc-37007) and FOXP3 siRNA (sc-43569) were purchased from Santa Cruz Biotechnology Inc. Sequence (5′ to 3′) of siFOXP3: #1 5’-AAGCAGCGGACACTCAATGAG-3’; #2 5’-AATGAGATCTACCACTGGTTC-3’.

Techniques: Biomarker Discovery, Western Blot, Knockdown, CCK-8 Assay, DNA Synthesis

Journal: American Journal of Cancer Research

Article Title: Overexpression of glucocorticoid receptor promotes the poor progression and induces cisplatin resistance through p38 MAP kinase in cervical cancer patients

doi:

Figure Lengend Snippet: Determining GR or FoxP3 expression in cervical cancer specimen. A tissue microarray (TMA) containing 188 cervical cancer, 218 high-grade CIN, 102 low-grade CIN specimens, and 300 non-adjacent normal cervical epithelia were stained to confirm the expression of GR or FoxP3. However, due to loss of spot while sectioning, 101 cervical cancer, 190 high-grade CIN, 36 low-grade CIN specimens, and 294 nonadjacent normal cervical epithelia were able to be interpreted for GR and 86 cervical cancer, 199 high-grade CIN, 48 low-grade CIN specimens, and 41 nonadjacent normal cervical epithelia were able to be interpreted for FoxP3. A. Representative immunohistochemical staining images of GR or FoxP3 (Scale bar 50 µm). B, C. Boxplots of IHC scores. The IHC staining score of GR or FoxP3 was significantly increased in cervical cancer specimens. In addition, their expression levels were significantly overexpressed in advanced FIGO stage.

Article Snippet: Dexamethasone and SB203582 was obtained from Sigma-Aldrich (St. Louis, MO) and Selleck Chemicals (Houston, TX). siRNA transfection GR and FoxP3 targeting specific small interfering RNAs (siRNAs) were commercially purchased from Santa Cruz Biotechnology (Santa Cruz, CA). siRNA was transfected into 6-wells plates using Lipofectamine ® RNAiMAX Reagent (Invitrogen, Gaithersburg, MD) at a dose of 50 pmol per well according to the manufacturer’s instructions.

Techniques: Expressing, Microarray, Staining, Immunohistochemical staining, Immunohistochemistry

Journal: American Journal of Cancer Research

Article Title: Overexpression of glucocorticoid receptor promotes the poor progression and induces cisplatin resistance through p38 MAP kinase in cervical cancer patients

doi:

Figure Lengend Snippet: Association of GR and FoxP3 expression with clinicopathological characteristics in human cervical neoplasia

Article Snippet: Dexamethasone and SB203582 was obtained from Sigma-Aldrich (St. Louis, MO) and Selleck Chemicals (Houston, TX). siRNA transfection GR and FoxP3 targeting specific small interfering RNAs (siRNAs) were commercially purchased from Santa Cruz Biotechnology (Santa Cruz, CA). siRNA was transfected into 6-wells plates using Lipofectamine ® RNAiMAX Reagent (Invitrogen, Gaithersburg, MD) at a dose of 50 pmol per well according to the manufacturer’s instructions.

Techniques: Expressing, Diagnostic Assay

Journal: American Journal of Cancer Research

Article Title: Overexpression of glucocorticoid receptor promotes the poor progression and induces cisplatin resistance through p38 MAP kinase in cervical cancer patients

doi:

Figure Lengend Snippet: Kaplan-Meier survival curve of GR or FoxP3 expression in cervical cancer specimen. A-F. Kaplan-Meier plot indicating the disease-free survival or overall survival categorized by GR or FoxP3 expression in patients with cervical cancer. G. The bar graph indicating the response to chemoradiation therapy categorized by GR or FoxP3 or combination of GR and FoxP3 expression in patients with cervical cancer. H. The correlation between GR and FoxP3 expression in patients with cervical cancer in our study samples. I. The correlation of GR with FoxP3 expression estimated by TIMER 2.0 in patients with cervical cancer.

Article Snippet: Dexamethasone and SB203582 was obtained from Sigma-Aldrich (St. Louis, MO) and Selleck Chemicals (Houston, TX). siRNA transfection GR and FoxP3 targeting specific small interfering RNAs (siRNAs) were commercially purchased from Santa Cruz Biotechnology (Santa Cruz, CA). siRNA was transfected into 6-wells plates using Lipofectamine ® RNAiMAX Reagent (Invitrogen, Gaithersburg, MD) at a dose of 50 pmol per well according to the manufacturer’s instructions.

Techniques: Expressing

Journal: American Journal of Cancer Research

Article Title: Overexpression of glucocorticoid receptor promotes the poor progression and induces cisplatin resistance through p38 MAP kinase in cervical cancer patients

doi:

Figure Lengend Snippet: Univariate and multivariate analyses of disease free survival or overall survival in cervical cancer patients

Article Snippet: Dexamethasone and SB203582 was obtained from Sigma-Aldrich (St. Louis, MO) and Selleck Chemicals (Houston, TX). siRNA transfection GR and FoxP3 targeting specific small interfering RNAs (siRNAs) were commercially purchased from Santa Cruz Biotechnology (Santa Cruz, CA). siRNA was transfected into 6-wells plates using Lipofectamine ® RNAiMAX Reagent (Invitrogen, Gaithersburg, MD) at a dose of 50 pmol per well according to the manufacturer’s instructions.

Techniques:

Journal: American Journal of Cancer Research

Article Title: Overexpression of glucocorticoid receptor promotes the poor progression and induces cisplatin resistance through p38 MAP kinase in cervical cancer patients

doi:

Figure Lengend Snippet: Knockdown of FoxP3 expression inhibits proliferation of cervical cancer cells. A. GR, FoxP3, and β-actin expression in Caski, and SiHa cells were analyzed by western blot. B. FoxP3 mRNA expression level was measured by qRT-PCR after constructing FoxP3 knockdown in Caski and SiHa cells. C. The proliferation of siControl- and siFoxP3-transfected Caski and SiHa cells was detected using EZ-Cytox assay, at every time points indicated. D. Colony formation assay with siControl- and siFoxP3-transfected cells were performed. Left panel: representative image of colonogenic assay. Right panel: quantitative results of colonogenic assay. The numbers of asterisks (*) indicate the level of significance: **P < 0.05, ***P < 0.005. Error bars represent mean ± standard error (S.E) of triplicate experiments.

Article Snippet: Dexamethasone and SB203582 was obtained from Sigma-Aldrich (St. Louis, MO) and Selleck Chemicals (Houston, TX). siRNA transfection GR and FoxP3 targeting specific small interfering RNAs (siRNAs) were commercially purchased from Santa Cruz Biotechnology (Santa Cruz, CA). siRNA was transfected into 6-wells plates using Lipofectamine ® RNAiMAX Reagent (Invitrogen, Gaithersburg, MD) at a dose of 50 pmol per well according to the manufacturer’s instructions.

Techniques: Knockdown, Expressing, Western Blot, Quantitative RT-PCR, Transfection, Colony Assay